If you're having account issues, try recovering your Intuit account information by visiting the Intuit account recovery page. You must hear the services from IVR that you may. ScanGuard Antivirus Support Contact (151O-37O-1986) Customer Service Number. Ccleaner Technical Support 151O-37O-1986 Phone Number Ccleaner Customer Service. To continue the call, you need to choose a language. CHARTER Customer Service +1- (8OO)- 541-O661 Technical Support Phone Number asif usa. You can also chat with our Mint support team. Dial the Turbotax contact number 1-800-4INTUIT (1-80) / (1-60). You can get assistance by leaving a message with our TurboTax or QuickBooks support team. Your ONLY source of information about your refund now is the IRS. Support links for TurboTax, QuickBooks, Mint, and Intuit accounting products. TurboTax does not receive any updates from the IRS. Make changes to your 2022 tax return online for up to 3 years after it has been filed and accepted by the IRS through. Included with TurboTax Deluxe, Premier, Self-Employed, Premium, TurboTax Live, TurboTax Live Full Service, or with PLUS benefits. Once your federal return has been accepted by the IRS, only the IRS has any control. Easy Online Amend: Individual taxes only. Get help signing in to your TurboTax Online account to start your tax return with TurboTax 1 best-selling tax software. TurboTax does not mail your tax return for you. Note: If it says “Ready to Mail” or “Printed” that means YOU have to mail it yourself. Or does your account say “Ready to Mail?” If the IRS accepted it, what does it say here? If you e-filed a state return, there will be a third email to tell you if the state accepted or rejected your state return.įirst, check your e-file status to see if your return was accepted: The second email will tell you if your federal return was accepted or rejected. The first one will say that your return was submitted. Some consumers who used Intuits TurboTax could get up to 85 each as part of a 50-state settlement over the software makers tactics that allegedly tricked. Depending on where you live, there are a few different ways you can contact us with privacy questions. When you e-file your federal return you will receive two emails from TurboTax. One here knows the status of your tax refund.
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In May of 2019, Binance was the victim of a large-scale hack. Via Simplex’s software, users can now buy cryptos like Bitcoin, Binance Coin, and Ethereum with a Visa or Mastercard. The Binance-Simplex partnership allows users to buy cryptocurrencies on Binance’s site with their credit cards. Simplex is a FinTech company that provides guaranteed fraudless payment processing. Simplex PartnershipĪt the start of the year, Binance partnered with Simplex as a way to get involved in the credit card space. The company then launched Binance Uganda, with similar goals for the African continent. Binance Jersey works as an independent entity from, its parent company. This would operate as a fiat-crypto exchange on the British island of Jersey. 2019Ĭontinuing their island expansion, Binance opened Binance Jersey. Founders Bank works to help crypto and blockchain-related firms secure financial services from traditional banks. Binance also purchased a significant stake in Founders Bank, a Maltese financial institution. In September of 2018, Binance teamed up with the Malta Stock Exchange to enable trading of security tokens. They would then invest an additional $5 million into blockchain startups.īinance also announced that they would help the government of Bermuda to develop a regulatory framework for blockchain and cryptocurrencies. They also announced that they would donate $10 million toward tech-related educational programs through their Binance Charity Foundation. In mid-2017, Binance announced that they would be building their global compliance center in Bermuda. They planned to develop relationships with small island nations where they could build offices and set up partnerships. It also boasted 10 million worldwide users.Īs Binance continued to grow, the company entered a phase of aggressive expansion. As a new platform in the crypto space, Binance was able to quickly mobilize out of China and continue to scale its business while other companies were still figuring out how they would relocate.Īfter 6 months of being open for business, Binance had accrued a profit of $350 million, mostly from transaction fees. Post-Chinaīecause many exchanges were operating out of China at the time of the ban, Binance had a unique advantage. After about 165 days of operation, Binance had become the world’s largest cryptocurrency exchange with regards to traded value. Today, the exchange supports over 120 different coins.Ĥ5 days after went live, the platform had accumulated over 120,000 users with an average growth rate of 5,000 new registered users per day.Īlthough the company was originally headquartered in China, they soon had to relocate to Japan following China’s ban on crypto trading. 11 days later, the Binance exchange opened.Īt the opening of the exchange, the only cryptocurrencies available on Binance were BNB, BTC, NEO, LTC, and ETH. On June 24, 2017, after raising $15 million, CZ’s Binance Coin started its initial coin offering with 200 million tokens. In 2017, CZ noticed the sudden rise in coin offerings and decided to make a coin of his own. He even worked as head of development at for 8 months. He followed this by creating software for futures trading at Bloomberg’s Tradebook.įinding the work unsatisfactory, CZ left the stock market space and bounced around various crypto projects. A few years later, he graduated with a degree in computer science from Montreal’s prestigious McGill University.Īfter college, CZ helped to build a system designed to match trade orders on the Tokyo Stock Exchange. After the government exiled his family for being part of the intellectual class, the Zhao’s moved to Vancouver, Canada in the 80s, when CZ was a teen.ĬZ spent his pre-college years working at McDonald’s and doing other odd jobs to help relieve his family’s financial woes. Binance’s founder, Changpeng Zhao, known as CZ, was born in Jiangsu, China. Binance’s History and Humble Beginningsīefore we go into a history of Binance, let’s learn more about its creator. With a single BNB coin originally costing 10 cents, nearly two years later one token has a value of $39.57.Ĭurious how Binance went from an obscure Chinese crypto exchange to the world’s premier platform? Continue reading and we’ll walk you through a history of Binance and look at how they got to be the cryptocurrency powerhouse they are today. Since its initial coin offering (ICO) in July of 2017, the BNB market cap has risen to over $4.5 billion. Binance Coin (BNB) is the cryptocurrency that powers the Binance exchange. Updated with the results of all games through October 3, 2021. As that game featured a home run, a double, and a single, and the Mariners/Dodgers games featured just a double and two singles, it broke the record for fewest total bases in a regular-season interleague game. In the Mariners' 1–0 loss to the Los Angeles Dodgers on April 20, the two teams combined for three hits, tying the record for the fewest in an interleague game set by the Boston Red Sox and Montreal Expos in 1997. This season was the second time during their drought that they’d won two more regular season games than the World Series champions. Despite posting a 90–72 record, their best since the 2003 season, the Mariners were eliminated from postseason contention for the 20th consecutive season after Boston swept the Washington Nationals to clinch the wild card berth and the Yankees clinched the remaining wild card spot on the last day of the season. Seattle is the only current Major League Baseball (MLB) franchise without a World Series appearance. The M's entered this season with the longest active playoff drought in the four major North American professional sports leagues, attempting to make their first postseason in twenty years. Seattle Mariners, t1 b1 t2 b2 t3 b3 t4 b4 t5 b5 t6 b6 t7 b7 t8 b8 t9 b9 SEA 50 DET. The Mariners played their 22nd full season (23rd overall) at T-Mobile Park, their home ballpark in Seattle, Washington. A 2021 Seattle Mariners schedule with dates for every regular season game played, opponents faced, a final score, and a cumulative record for the 2021 season. The 2021 Seattle Mariners season was the 45th season in franchise history. MLB Scores & Boxes 2021 MLB Scores & Schedule Toronto Blue Jays Schedule Seattle Mariners Schedule Schedules, Scores, & Boxes. Visit for the complete box score, play-by-play, and win probability. ( Rick Rizzs, Aaron Goldsmith, Dave Sims) Seattle Mariners beat Toronto Blue Jays (9-7). The new campaign starts Thursday, and the league is returning to a full, 162-game schedule for the 2021 season. ( Dave Sims, Aaron Goldsmith, Mike Blowers) Getty Images Major League Baseball Opening Day 2021 is here. RHP Sonny Gray (4-4, 3.15 ERA) is expected to start for the Twins against Royals RHP Brady Singer (6-8, 5.55 ERA).Baseball Club of Seattle, LP, represented by CEO John Stanton Twins: After flying out Wednesday evening, Minnesota will spend an off day in Kansas City before six games in seven days in Missouri. The Diamondbacks have yet to announce a starting pitcher. RHP Logan Gilbert (8-5, 3.55 ERA) is scheduled to start Friday for Seattle. Mariners: Off Thursday ahead of a three-game series at Arizona. Joey Gallo left Wednesday’s game due to illness. Buxton has been on the paternity list since he and his wife celebrated the birth of their third child. Baldelli expects Polanco, who has been out since June 9 with a left hamstring injury, to be activated during the trip.ĭH Byron Buxton will also rejoin the team for the series against the Royals. Paul, then join Minnesota for its six-game road trip to Kansas City and St. INF Jorge Polanco was scheduled to make his final rehab appearance Wednesday in Triple-A St. Kiriloff will likely return for Minnesota’s series at Kansas City. Manager Rocco Baldelli said Kirilloff tweaked the shoulder on a diving play in the past week. Twins: A day after being named AL player of the week, 1B/OF Alex Kirilloff was scratched from the lineup Wednesday with a sore shoulder. Seattle also transferred LHP Marco Gonzales to the 60-day injured list to clear a 40-man spot for Thornton. McCoy, 29, spent the past two seasons at Tacoma. One-year: Batting - 95, Pitching - 96 Pythagorean W-L: 76-86, 697 Runs, 748 Runs Allowed Run Differential: -51 Fun Differential: +90 Become a Stathead & surf this site ad-free. The Mariners said Thornton would be optioned to Triple-A Tacoma. Seattle Mariners, t1 b1 t2 b2 t3 b3 t4 b4 t5 b5 t6 b6 t7 b7 t8 b8 t9 b9 SEA 50 BAL. What was the longest win streak for the 2021 Seattle Mariners The longest. Thornton has appeared in 108 games over parts of five seasons with the Blue Jays, including 37 appearances in 2021 and 32 last year. During the regular season, the 2021 Seattle Mariners went 13-7 in extra inning games. Thornton has appeared in four games this season for the Blue Jays, allowing one earned run in 5 1/3 innings pitched. Seattle acquired RHP reliever Trent Thornton from Toronto in exchange for minor league IF Mason McCoy. Founded in 1977, the Mariners compete in Major League Baseball (MLB) as a member club of the American League (AL) West division. He surrendered one earned run and struck out four batters. The Seattle Mariners today announced an updated 2021 Cactus League schedule featuring 14 home games at the Peoria Sports Complex and 14 road games (including one against the San Diego Padres at Peoria) for a total of 28 Spring Training games. Paul on Wednesday, reliever Josh Winder gave a depleted Twins bullpen four innings of relief. Whether you’re new to coloring or experienced, you’ll find much to enjoy in this high quality coloring book. On an artistic level, as the title suggests, the theme is the wonders of childhood memories, with thirty pages of artistic borders with type to color, followed by the individual themes, which include butterflies, tea party, kitties, playing, the beach, and many more. The pages are one-sided and heavy enough to prevent bleed through with pencils and water-based markers. The drawings are centered on the page with enough white space on the edges so the pattern is not the page creases. The patterns are challenging enough to provide interest but yet not so detailed that it turns a relaxing activity stressful. I’m a failure at traditional meditation but when I pick up my pencils and markers and start coloring it quiets my mind.Īs with her first coloring book, Color in Fashion, Alecia Blake’s artistic experience and talent is highlighted in these pages. There’s something calming and meditative about coloring. Many an eye, now dim, has gazed on them in silent wonder and admiration. Find calmness and peace while you color these pages and make them your own. I presented her with a book which I happened to have about me, and should not. I hope that coloring these pages takes you away from the pressures of everyday life into the fun and whimsical world of child-like dreams and memories. My wish is that this book full of original, intricate hand-drawn illustrations depicting memories of childhood will trigger your own memories and bring you creative inspiration and relaxation. Drawing children provided me with so much pleasure that it gave me the idea to create this book, and I am so excited to share it with you. Colored pencils, crayons and water-based markers are best, but if using alcohol-based markers, slip a blank white sheet of paper behind each page for added protection.My extensive background as an illustrator included doing many children's illustrations for various publications. Colorists will enjoy varied, intricate backgrounds, full of country, forest, beach and playground scenes, floral patterns, toys, dolls, butterflies, kitties, and much, much more.Each coloring page is printed on a separate sheet to avoid bleed through. It is filled with 30 pages of elaborate borders with color-in type and illustrations full of the wonder of childhood memories. Includes: 18 Color Wonder Coloring Pages 5 Color Wonder Markers 12" x 9" Bluey Bluey Gifts: Books Gifts: Gifts for Her Gifts: Gifts for Him Holiday: Stocking Stuffers New to the Shop Shop by Price: Gifts Under $25 Summer '23 Catalog Summer Kick Off add-to-cart ///cdn/shop/products/23955C.jpg?v=1678461397 ///cdn/shop/products/23955B.jpg?v=1678461396 40780763562032 Default Title 13.98 13.98 ///cdn/shopifycloud/shopify/assets/no-image-2048-5e88c1b20e087fb7bbe9a3771824e743c244f437e4f8ba93bbf7b11b53f7824c.Welcome to Color in Wonder: An Adult Coloring Book of Childhood Memories! This adult coloring book is for kids at heart (like me!). Makes a great gift for girls and boys ages three and upĭimensions: 0.75 inches length x 9 inches width x 11.938 inches height The convenient "foldalope" packaging works as reusable storage for your coloring pages and markers. This set contain 18 fun-filled coloring pages depicting silly scenes featuring Blippi and his play-and-learn adventures! Each page works with all Color Wonder markers, paints, and stamps. Kids can color their favorite, fun-loving friend, Blippi, in a mess-free way with Crayola Color Wonder! Color Wonder colors appear only on special Color Wonder paper, not on skin, clothing, or carpets. 7202382676016 Bluey: Color Wonder Coloring Set 13.98 ///cdn/shop/products/23955A.jpg?v=1678461397 ///cdn/shop/products/23955A_large.jpg?v=1678461397 USD InStock Books Bluey Books Gifts for Her Gifts for Him Gifts Under $25 New to the Shop New Toys & Games Stocking Stuffers Summer '23 Catalog Summer Kick Off Sale Also have some that are wmv but will freeze my windows media player if I jump the video back and forth. Some are in MP4 and does not play and others in this format do. I have some porn my system has trouble playing. I have a nice Samsung 24" monitor running at 1920x1080 Youtube download and convert was great - quality was amazing for what it was - no blocky looking output - all smooth and even looked ok at full screen. 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Download videos from YouTube and convert them to desired formats.Broad video and audio formats are supported to convert to or from like AVI, Xvid, DivX, H.264, MP4, 3GP, MKV, WMV, RM, FLV, MOV, WebM, WTV, MP3, AAC, AC3, WAV, WMA, FLAC, Apple Lossless, AIFF, APE, etc. Bigasoft Total Video Converter provides professional solutions for various video conversion, audio conversion and video to audio conversion, as well as advanced features like trim, crop, merge, split videos and more. One involves working with local businesses to revitalize the street. The mayor said he has spoken with Welch and hopes that he will keep his business in Minneapolis.Īs for West Broadway, Frey said he's got plans in his budget for economic development and public safety. On Wednesday, Frey said although the city has seen a drop in violent crime citywide, "the improvement hasn't come fast enough." The gunfire prompted Mayor Jacob Frey to say Monday he's redoubling efforts to curb shootings and other violent crimes. Ten people were shot, four of them fatally, including the shooting at Cliff & Norm's bar, next door to Boom Island. Last weekend was exceptionally violent in Minneapolis. "But I'm scared to walk out to my own car." "We believe in the neighborhood and wanted to make the most out of it," he said. Sometimes he'll nap on the taproom floor while he waits for them to scatter. They often physically block Boom Island's gate at night with their vehicles, he said, preventing him from leaving after-hours. Welch said he began walking employees to their cars after biker gangs started peddling drugs in the alley. Unlike most bars, Boom Island closes each weekend by 9 p.m. Last February, someone smashed out the car windows of regular customers two weeks in a row. He’s been an employee of Boom Island Brewing for five years. Taproom manager Jim Barnard, of Robbinsdale, poured a beer for a customer Tuesday.
Step-by-step tutorial here instructables Make a Pallet Wood Clock:Īnother great and fantastic wood clock to make in no time. A wood stain will be a great choice to create a dark choco tone of wood. Just put together the wooden squares of equal dimensions making a large square and install a clock mechanism to the center square. DIY Wooden Clock in 8 Steps:īring the warmth of to any room ambiance with this another graceful design of clock, will whip up quickly in 8 easy steps. See the entire list of amazing DIY clock ideas and designs and click on the attached links to grab full free tutorial. Next, spread the decorative fabric on the wall and then add the decorative plastic wall art frames as numerals! Install the clock kit in the center to make an extraordinary decorative wall clock. Make the play do lid clocks for the kids’ rooms that will be colorful and enchanting. Your teans will also love these DIY clocks for their bedrooms so let your kids try these crafts for teens.Ĭover also the plywood circles with colorful fabric and make round fabric wall clocks in no time. Install the clock kits to your home plates to make gorgeous plate wall clocks will surely lure the number of views of your home walls. Mount the mini planter bucket and garden tool toys as numerals on a wooden round and make the faux garden wall clocks. And also bring the amazing rustic decor vibes to your interior wall by hanging a mid-century modern Esque starburst wall clock, will be a big delight to view for sure. Bring the modern geometric decor appeal to your home interior walls by making fat quarter hexagon wall clocks. Get rid of typical old wall clocks and make the circle wood clock that may come decorative with custom elements and numerals. Here most of these wall clocks are made of recycled materials. Along with being a lovely indicator of time, these DIY clocks are also going to be the best decorative elements for your home interior design. So, if you want to turn your best art into a clock then explore this big list of 75 best DIY clock ideas to make your own clocks at home.Īll these DIY clock ideas are homemade and affordable and are sure to bring great decorative value to any targeted interior wall. The DIY clocks are still popular, and they make the perfect wall art if it comes in decorative appeal. But some things get never changed like the decorative value of the clocks in the interior decor. Do you want to customize your own clock? If Yes, then you’ll learn here about how to make your own custom wall clock at home? Now all the old living styles and trends have been changed as we use mobile phones and tech gadgets to know the time instead of using the traditional clocks and watches. A eukaryote cell is shown, but the same methods can be applied to prokaryotes, too. A marker lane is shown in the left of each gel to determine size. DNA is in blue, RNA in red, and polypeptides in green. Size and amount of DNA, RNA, and polypeptides can be determined using similar blotting methods. An alternative name for the technique is Cleaved Amplified Polymorphic Sequence (CAPS) assay.\): Comparison of Southern, Northern, and Western blots. Northern blotting was developed by Jamse Alwine and George Stark at Stanford University and was named such by analogy to Southern blotting. Northern Blotting Northern blotting is a technique for detection of specific RNA sequences. Therefore, more samples can be analyzed in a shorter time. Azhar Chishti Example of Application of SB in diagnosis of mutation in globin gene 2. However, PCR can be used to amplify very small amounts of DNA, usually in 2-3 hours, to the levels required for RFLP analysis. Isolation of sufficient DNA for RFLP analysis is time consuming and labor intensive. 23456 7 89 Steps of Southern blot include restriction digests of DNA. Typically, in species with moderate to high polymorphism rates, two to four restriction endonucleases are used such as EcoRI Since its development in 1975, 1 Southern blot analysis has been used in various research and applications. The probes are screened for RFLPs using genomic DNA of different genotypes digested with restriction endonucleases.Southern blots of the inserts can be probed with total sheared DNA to select clones that hybridize to single- and low-copy sequences.Digests of the plasmids are screened to check for inserts.The digested DNA is size-fractionated on a preparative agarose gel, and fragments ranging from 500 to 2000 bp are excised, eluted and cloned into a plasmid vector (for example, pUC18). Southern blotting was applied in many important studies like the genetic mapping of the human genome which was based on blotting based detection of restriction fragment length polymorphisms.Total DNA is digested with a methylation-sensitive enzyme (for example, PstI), thereby enriching the library for single- or low-copy expressed sequences (PstI clones are based on the suggestion that expressed genes are not methylated).SNPsor INDELs can create or abolish restriction endonuclease (RE) recognition sites, thus affecting quantities and length of DNA fragments resulting from RE digestion. The RFLP probes are frequently used in genome mapping and in variation analysis (genotyping, forensics, paternity tests, hereditary disease diagnostics, etc.). southern blotting, northern blotting & western blotting, mechanism of. Short, single- or low-copy genomic DNA or cDNA clones are typically used as RFLP probes. Analytical uses of Enzymes in laboratory investigations (enzyme based assays). Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.Īn RFLP probe is a labeled DNA sequence that hybridizes with one or more fragments of the digested DNA sample after they were separated by gel electrophoresis, thus revealing a unique blotting pattern characteristic to a specific genotype at a specific locus. RFLP, as a molecular marker, is specific to a single clone/restriction enzyme combination. Is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases. Restriction Fragment Length Polymorphism (RFLP) Introduction Restriction Fragment Length Polymorphism (RFLP) |
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